A graph-based spectral alignment algorithm that identifies glycopeptides with multiple glycosylation sites from tandem mass spectra.
Input: FASTA format peptide database file, and MGF format spectra data file
Execute:
Usage: ./GlycoMID [-para value] peptide_database MS/MS_file.mgfInput file: peptide database: FASTA Spectrum: MGF parameters: -E value: error tolerance for precursor mass, default = 0.05 -e value: error tolerance for fragment ions, default = 0.2 -a value: activation type [CID/ETD/HCD], default = CID -c value: maximum charge state of fragment ions, default = 2
Output: identified peptide-spectrim-match list